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Brain tumour samples were unmoving in 10% neutral buffered formalin (Sigma) and embedded indium paraffin wax. The psyche tumor samples were divided at 5  what is a good diet for fast weight loss μm, were painted with haematoxylin and bromeosin (H&E) at the Harvard University Rodent Histopathology Core Facility (Boston, MA), and were examined past light microscopy victimization either A Zeiss Axioplan 2 or Nikon SMZ1500 light microscope. Images were nonheritable exploitation SPOT Imaging Solutions (Diagnostic Instruments, Inc.) cameras and package. All microscopic anatomy sections were evaluated at the Harvard University Rodent Histopathology Core Facility. Immunohistochemistry: Ki-67 and Iba-1

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