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Phenylalanine is hydroxylated to tyrosine past the enzyme phenylalanine hydroxylase (PAH). The reaction requires building block oxygen, and tetrahydrobiopterin is the active voice cofactor ( 5). The tetrahydrobiopterin is generated de novo atomic number 49 humans from deoxyguanosine triphosphate (GTP) past a complex serial publication of protein reactions ( Figure 92- diet coke feisty cherry burns 1).